HS-AFM image data were processed by a laboratory-made software program written in Igor Pro (WaveMetrics, Lake Oswego, OR, USA). The number of pixels of each HS-AFM image was increased twice by linear interpolation. The molecule of interest was tracked to compensate for drift of sample position. The tracking was conducted by calculating a coefficient of correlation between a template image and each frame image under various lateral shifts of the latter image. The lateral shift providing the maximum coefficient value was adopted. Successive HS-AFM images were stacked and averaged, where frames with unclear images were removed.

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