Purified samples were dispensed on glow-discharged carbon-coated copper grids. Each grid was negatively stained with 2.0% (w/v) uranyl acetate. Micrographs were collected on a direct electron detector (FEI Falcon II) using FEI Tecnai G2 F20 (FEI, Hillsboro, OR, USA) operating at 200 kV. For reference-free 2D class averaging, particle images were boxed out with e2boxer.py in the EMAN2 package (29) and then processed with RELION software (30).

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.