α-SNAP–dependent binding of NSF to the maltose-binding protein (MBP)-SNARE complex assay and the MBP-SNARE complex disassembly assay
This protocol is extracted from research article:
Mechanistic insights into the SNARE complex disassembly
Sci Adv, Apr 10, 2019; DOI: 10.1126/sciadv.aau8164

The α-SNAP–dependent binding of NSF to the SNARE complex assay and the SNARE complex disassembly assay was performed as described before (30, 53). At least three independent experiments were carried out on at least two different batches of purified proteins.

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