Contiguity of hotspots across biogeographic realms
This protocol is extracted from research article:
Multiple macroevolutionary routes to becoming a biodiversity hotspot
Sci Adv, Feb 6, 2019; DOI: 10.1126/sciadv.aau8067

We determined whether different spatial clustering of hotspot and non-hotspot areas within each realm could influence our results. For each realm, we calculated the median distance of each cell to all neighboring cells of the same class (hotspot or non-hotspot) with a radius of 1000 km (fig. S6). We then compared the differences of the mean distances among hotspots and non-hotspots using a t test.

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