Raw sequencing reads were first processed to remove adaptors and low-quality bases using Trimmomatic (v. 0.33). Then, clean reads were mapped to human genome GRCh38 (from ENSEMBL) using STAR (020201) with default parameters. Raw counts generated by STAR were concatenated together and fed to DEseq2 (1.16.1) to get normalized expression and screen DE genes. Threshold for DE genes are false discovery rate less than 0.1 and absolute fold change larger than 1.5. Gene ontology enrichment analysis was performed using GOEAST. All plots were generated using www.ehbio.com/ImageGP.

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