The ChIP assay was performed using the EZ-ChIP Kit from Millipore according to the manufacturer’s instruction. The primers used for the ChIP assay were as follows:

mouse RANKL, 5′-GAGTTCTAGAATTTCCCCAAG-3′ and 5′-GTGCCTCTTTCACTCCCACAT-3′ (48); mouse TNF-α, 5′-TGTACCGCAGTCAAGATATG-3′ and 5′-TGCCTGTGTCTATTTCCTTT-3′.

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