Casflox/flox and Relaflox/+ mice of C57BL/6 background (35, 36) were mated with Dmp1-Cre transgenic mice, in which the Cre recombinase gene was specifically expressed in osteocytes (37). Dmp1-Cre+/−, Casflox/flox (Cas cKO) and Dmp1-Cre−/− Casflox/flox (normal littermates) mice were generated by mating Dmp1-Cre−/−, Casflox/flox male mice with Dmp1-Cre+/−, Casflox/+ female mice. Dmp1-Cre+/−;Casflox/flox;Relaflox/+(Cas/Rela cKO) and Dmp1-Cre−/−;Casflox/flox;Relaflox/+ (normal littermates) mice were generated by mating Dmp1-Cre+/−;Casflox/flox;Rela +/+ male mice with Dmp1-Cre−/−;Casflox/flox;Relaflox/+ female mice. Dmp1-Cre+/–Relaflox/+ (Rela cKO) and Dmp1-Cre−/−;Relaflox/+ (normal littermates) mice were generated by mating Dmp1-Cre+/−;Rela +/+ male mice with Dmp1-Cre−/−;Relaflox/flox female mice. All animals were housed under specific pathogen–free conditions and treated with humane care under approval from the Animal Care and Use Committee of Tokyo Metropolitan Institute of Gerontology, National Rehabilitation Center for Persons with Disabilities, and Nadogaya Hospital. For all mouse analyses except for those at 1 day after birth (fig. S1), male mice were used.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.