For quantification of the Western blot data, ImageJ software was used to measure the relative intensity of each band, and the relative protein or modification levels were normalized to the relative loading control levels. Unless otherwise indicated, data are presented as the means ± SD from at least three independent experiments, and the differences between any two groups were compared by unpaired t tests. The data were scatter-plotted using the Prism 5 software. *P < 0.05, **P < 0.01, ***P < 0.001, and ns indicates “not significant.”

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