Preparation of chromatin fractions was as described previously with minor modifications (40). Briefly, 1 hour after treatment with VP-16, cells were washed three times with PBS and maintained with fresh medium without VP-16 for 15 hours. Cells were then collected, and cell pellets were subsequently resuspended in buffer [20 mM tris-HCl, (pH 8.0), 100 mM NaCl, 1 mM EDTA, and 0.5% NP-40] and incubated on ice for 20 min. Nuclei were then collected and resuspended in HCl (0.2 M). The soluble fraction was neutralized with tris-HCl (1 M at pH 8.0) for further analysis.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.