Preparation of chromatin fractions was as described previously with minor modifications (40). Briefly, 1 hour after treatment with VP-16, cells were washed three times with PBS and maintained with fresh medium without VP-16 for 15 hours. Cells were then collected, and cell pellets were subsequently resuspended in buffer [20 mM tris-HCl, (pH 8.0), 100 mM NaCl, 1 mM EDTA, and 0.5% NP-40] and incubated on ice for 20 min. Nuclei were then collected and resuspended in HCl (0.2 M). The soluble fraction was neutralized with tris-HCl (1 M at pH 8.0) for further analysis.

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