pX459-G9a-sgRNAs were transfected into HeLa S3 cells. pX260-Plk1-sgRNA and pMD19-Plk1-K209M/A were transfected into HeLa/RFP-H2B cells. Then, the edited cells were selected by adding puromycin (1 μg ml−1). The positive clones were collected and plated onto 96-well plates at a concentration of approximately one cell per well. One week later, the single-colony cells were selected for validation using a combination of Western blot assay and genomic sequencing.

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