For Fig. 2C, 2 μg of recombinant GST-tagged N-Plk1 was incubated overnight with/without an equal amount of recombinant GST–G9a-SET at 30°C in the presence of 2 μCi of 3H-labeled SAM. After that, a half volume of reaction mixture was examined by scintillation counting to verify the methylation of N-Plk1. The other half of mixture was added with 200 μM cold ATP and 0.5 μg of GST–Aurora A with buffer [25 mM tris-HCl, 10 mM MgCl2, 2.5 mM DTT, 0.5 mM EGTA, and 0.1 μg μl−1 bovine serum albumin (BSA)] and reacted for an extra 1 hour. Reactions were stopped with 2× SDS sample buffer and examined by Coomassie staining or autoradiography.

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