The following antibodies and reagents were obtained from commercial sources: The α-Plk1 (F-8) (sc-17783), α-HA (Y-11) (sc-805), and α-Wee1 (B-11) (sc-5285) antibodies were purchased from Santa Cruz Biotechnology; α-Flag (F7425-2MG) and α-Aurora A (A1231) antibodies were purchased from Sigma-Aldrich; α-G9a/EHMT2(C6H3) (3306S) and α-pCdc2 (Y15) (4539T) antibodies were purchased from Cell Signaling Technology; α-cyclin B1 (1495-1), α-phospho histone H3 pS10 (1173-1), and α-phospho Plk1 pT210 (3646-1) antibodies were purchased from Epitomics; α-BubR1 (ab172581), α-RAD51 (ab133534), and α-H3K9me2 (ab1220) antibodies were purchased from Abcam; α-eGFP (50430-2-AP), α-RPA2 (10412-1-AP), α-Myc (60003-2-Ig), and α-Actinβ (60008-1-Ig) antibodies were purchased from Proteintech; α-H3K9me2 (A2359) and mouse α-GAPDH (glyceraldehyde phosphate dehydrogenase) (AC002) antibodies were purchased from ABclonal; α-H3 (39163) antibody was purchased from Active Motif; pan-mono/dimethyl (TM602) antibody was purchased from PTM Biolabs; α-HEC1(9G3.23) (GTX70268) antibody was purchased from GeneTex; α-Plk1 K209me1 antibody was generated by ABclonal by immunizing rabbits with a K209-monomethylated peptide (DGERKK(me1)TLC) conjugated with keyhole limpet hemacyanin; secondary horseradish peroxidase–conjugated α-mouse or α-rabbit antibodies were purchased from Jackson ImmunoResearch Laboratories; the α-FLAG M2 affinity gel (A2220), thymidine, and nocodazole were purchased from Sigma-Aldrich; the α–hemagglutinin (HA) affinity gel (B23301) was purchased from BioTool; BIX-01294 was purchased from Selleck; VP-16 and VE-821 were purchased from TargetMol; and CPT was purchased from Aladin.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.