Commercially available needle-type oxygen microsensors (Oxygen Microsensor; NTH-Pst1) and a microfiber optic oxygen transmitter (Microx TX3) from PreSens were used to measure in vivo O2. After 12 hours, needle-type sensors, controlled by a micromanipulator and concurrent with temperature readings using a manufacturer-provided thermometer, were inserted into the hydrogel, and O2 was recorded when the reading stabilized. All animal studies were performed following approval by the Johns Hopkins University Institutional Animal Care and Use Committee (MO15A154, MO18A244).

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