Following ECFC encapsulation in Gel-HI, ECFC medium (EGM2) was supplemented with exogenous MMP (collagenase type IV; Thermo Fisher Scientific) at a range of concentrations. Bright-field images were captured at predetermined time points to monitor cell morphology (Olympus IX50). ImageJ (public domain) and a custom MATLAB script (MathWorks) were used to quantify cell clusters. Graphs were generated using GraphPad Prism 6 (GraphPad Software Inc., La Jolla, CA).

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