Following ECFC encapsulation in Gel-HI, ECFC medium (EGM2) was supplemented with exogenous MMP (collagenase type IV; Thermo Fisher Scientific) at a range of concentrations. Bright-field images were captured at predetermined time points to monitor cell morphology (Olympus IX50). ImageJ (public domain) and a custom MATLAB script (MathWorks) were used to quantify cell clusters. Graphs were generated using GraphPad Prism 6 (GraphPad Software Inc., La Jolla, CA).

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.