ECFCs were transfected with SMARTpool:siGENOME HIF1A and Non-Targeting siRNA 1 (scr) according to the manufacturer’s protocol and previous literature (31). Briefly, cells were seeded on a six-well plate and treated with 50 nM siRNA. We confirmed knockdown via quantitative real-time fluorescence polymerase chain reaction after 24 hours and used transfected cells in experiments. ImageJ (public domain) was used to quantify cell clusters. Graphs were generated using GraphPad Prism 6 (GraphPad Software Inc.).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.