ECFCs were transfected with SMARTpool:siGENOME HIF1A and Non-Targeting siRNA 1 (scr) according to the manufacturer’s protocol and previous literature (31). Briefly, cells were seeded on a six-well plate and treated with 50 nM siRNA. We confirmed knockdown via quantitative real-time fluorescence polymerase chain reaction after 24 hours and used transfected cells in experiments. ImageJ (public domain) was used to quantify cell clusters. Graphs were generated using GraphPad Prism 6 (GraphPad Software Inc.).

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