Lentiviral constructs with shRNAs were directed against murine Ms4a6d, A20, and Stat3 in the pGV112 vectors. The primers are shown in table S1. Lentivirus was prepared by transient transfection of 293T cells with transfer vectors along with third-generation packaging constructs (pHelper 1.0 and pHelper 2.0). The viral titers were determined with serial dilution of virus-containing media on NIH3T3 cells. RAW264.7 cells or PEMs were transfected with unconcentrated virus supernatant overnight in the presence of polybrene (8 mg/ml) and selected in puromycin (0.5 mg/ml).

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