For simultaneous pH measurements by SNARF-1 and a microsensor, microsensor data were recorded continuously every 5 s, and pH using SNARF-1 was measured punctually every 5 to 10 min. A more frequent acquisition of fluorescent dye images was not possible because of the sensitivity of the coral to the laser and a related tissue retraction. Microsensor measurements were subsequently averaged for the specific time frames (110 s) of each SNARF-1 data acquisition.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.