For simultaneous pH measurements by SNARF-1 and a microsensor, microsensor data were recorded continuously every 5 s, and pH using SNARF-1 was measured punctually every 5 to 10 min. A more frequent acquisition of fluorescent dye images was not possible because of the sensitivity of the coral to the laser and a related tissue retraction. Microsensor measurements were subsequently averaged for the specific time frames (110 s) of each SNARF-1 data acquisition.

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