Gelatin-g–ferulic acid (Gtn-FA) was synthesized using EDC and NHS as coupling reagents. A mixture of DMSO and distilled (DI) water (1:1 volume ratio) was prepared as a solvent. Gtn (1.0 g) was dissolved in 50 ml of the solvent at 40°C. FA (0.777 g, 4.0 mmol) was dissolved in 20 ml of the solvent and reacted with EDC (0.92 g, 4.8 mmol) at room temperature for 15 min and then with NHS (0.64 g, 5.6 mmol) at room temperature to activate the terminal carboxyl groups of FA (carboxyl/EDC/NHS = 1:1.2:1.4). The activated solution was then added to the Gtn solution, and a conjugative reaction was conducted at 40°C for 24 hours. Following completion of the reaction, the solution was dialyzed against DI water for 5 days (molecular mass cutoff = 3500 Da) and then lyophilized.

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