The Vsig4-deficient (Vsig4−/−) mice were provided by M. van Lookeren Campagne (Department of Immunology, Genentech, CA, USA). Nlrp3−/− (#017970), Il-1R1−/− (#003245), and C57BL/6 WT were purchased from the Jackson laboratory (Bar Harbor, Maine, USA). The Ms4a6d−/− mice were generated by Cyagen Biosciences (Guangzhou, China) using the CRISPR/Cas9 technique (fig. S10). Vsig4−/− mice were crossed with Nlrp3−/− and Il-1R1−/− mice to develop Vsig4−/−Nlrp3−/− and Vsig4−/−Il-1R1−/− DKO mice. All mice were backcrossed 10 times onto the B6 background to avoid unpredictable confounders. Mice were maintained in microisolator cages, fed with standard laboratory chow diet and water, and housed in the animal colony at the animal center of the Third Military Medical University (TMMU). All animals received humane care according to the criteria outlined in the Guide for the Care and Use of Laboratory Animals prepared by the National Academy of Sciences and published by the National Institutes of Health (NIH publication 86-23, revised 1985). All of the experiments comply with the animal study protocol approved by the Laboratory Animal Welfare and Ethics Committee of the TMMU (no. SYXK-PLA-20120031).

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.