Cells were incubated for 30 min on ice with primary antibody diluted in 100 μl of primary antibody staining buffer per sample as indicated in table S1A. Cells were washed one time with cold PBS (p75-NGFR/HNK1 flow cytometry) or MACS buffer (NG2 and PDGFRβ flow cytometry). Cells were subsequently incubated in 100 μl of primary antibody staining buffer with 1:500 Alexa-tagged isotype-specific goat secondary antibodies. Cells were washed as previously described and resuspended in 4% PFA for 15 min at room temperature. Cells were subsequently stored in wash buffer for up to 24 hours at 4°C before running samples on a cytometer.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.