IMR90C4 and CS03n2 iPSCs and H9 hESCs were maintained on Matrigel-coated plates in E8 medium, which is Dulbecco’s modified Eagle’s medium (DMEM)/F12 basal medium supplemented with l-ascorbic acid-2-phosphate magnesium (64 mg/liter), sodium selenium (14 μg/liter), FGF2 (100 μg/liter), insulin (19.4 mg/liter), NaHCO3 (543 mg/liter), transferrin (10.7 mg/liter), and TGFβ1 (2 μg/liter) and prepared according to Chen et al. (74). When cells reached ~70% confluence, they were passaged using Versene to new Matrigel-coated plates. For hPSCs used in BMEC differentiations, cells were maintained in mTeSR1 on Matrigel plates and passaged as previously described (75).

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