BT549, CAL-120, and MDA-MB-468 cells were preincubated in DMEM-F12 or Plasmax (both supplemented with 2.5% FBS) for 2 days for the experiments reported in Fig. 1, and for all experiments, cells were seeded in six-well plates in 2 ml of medium. Cell densities, incubation times, and components added to the media are indicated in Figs. 1 and 2. Fresh medium was added after 96 hours to prevent nutrient exhaustion. At endpoint, colonies were fixed with trichloroacetic acid (final concentration of 3%, 30 min incubation at room temperature), washed with water, and stained with sulforhodamine B (0.057% in 1% acetic acid, 1 hour incubation at room temperature). LI-COR Odyssey and Image Studio 5 were used for image acquisition, and ImageJ was used for quantification of area covered by colonies and colony number. For the identification of sodium selenite as a stimulator of colony formation, MDA-MB-468 cells were cultured in a mixture of DMEM-F12 and Plasmax (1:1) and in DMEM-F12 supplemented with the different Plasmax stock solutions or individual Plasmax components reported in table S1.

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