Tissue sections were deparaffinized and submitted to antigen retrieval treatments, as previously described (43). After two blocking steps with 3% H2O2 (Sigma-Aldrich) for 10 min, and 10% horse serum (Vector) for 30 min, slides were incubated for 60 min at room temperature with the primary antibody (table S4). A secondary biotinylated antibody (Vector Labs) was applied for 30 min, followed by detection (30 min) with an avidin/biotin system (VECTASTAIN ABC Elite, PK-7100, Vector). The signal was revealed with Dako Envision system using DAB substrate (3,3′-diaminobenzidine; K5007, Dako). Nuclei were stained with hematoxylin (K8018, Dako) for 3 min. Isotype controls immunoglobulins (Vector) were included in all experiments to attest of the specificity of the staining. Sections were dehydrated in the Leica AutoStainer XL and mounted with DPX Mountant (VWR International).

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