Mice were euthanized by exsanguination under deep anesthesia. WT male and female mice were compared to hSyt2 mice. There were three mice per sex per group, and 51 tissue sections per animal were examined. Tissues were fixed in formalin or in Davidson’s solution (eyes, lacrimal and Harderian glands, testes, and epididymides) for 24 hours and then embedded in paraffin. Tissue sections were hematoxylin and eosin–stained, and additional testicular sections were stained using periodic acid–Schiff for a better examination. Tissue sections were examined by a board-certified veterinary pathologist.

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