The RT and RT-PCR were performed as described previously (39). Leaves of seedlings at 10 days old were used for RNA isolation, and oligo(dT) primers (Promega) were used for RT. The CFX real-time PCR machine (Bio-Rad) and SYBR Green mixture (Roche) were used for RT-PCR. The relative expression of the genes was measured relative to UBIQUITIN 10 or the input. Data file S1 lists the primer sequences.

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