Images were taken using a ZEISS V20 fluorescence stereomicroscope with AxioCam MRc or MRm camera and ZEN lite software (ZEISS). Regenerates were imaged while fully extended and moving to ensure the absence of any tissue bunching, which could affect analyses. Heat maps for visualizing intensity of ROS levels were generated using the standard rainbow lookup table (LUT) within the ZEN lite software. Adobe Photoshop was used to orient, scale, and improve clarity of images (but not for fluorescent images). Data were neither added nor subtracted; original images are available upon request.

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