Images were taken using a ZEISS V20 fluorescence stereomicroscope with AxioCam MRc or MRm camera and ZEN lite software (ZEISS). Regenerates were imaged while fully extended and moving to ensure the absence of any tissue bunching, which could affect analyses. Heat maps for visualizing intensity of ROS levels were generated using the standard rainbow lookup table (LUT) within the ZEN lite software. Adobe Photoshop was used to orient, scale, and improve clarity of images (but not for fluorescent images). Data were neither added nor subtracted; original images are available upon request.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.