The MagShield box was housed in a temperature-controlled room (20°C). The temperature inside the Helmholtz coils during magnetic field exposure assays was randomly tested twice a day over the 3-day assay, with an average temperature of 22°C (±1°C). Animals were placed in worm water in either 35 or 60 mm petri dishes at the indicated times into the MagShield box in the center of the partition (via a stack of larger plastic petri dishes). Animals were exposed to controlled static magnetic fields (in the dark) always in tandem: with one side of the MagShield partition set to Earth-normal 45 μT (for controls) and the other partition set to the indicated experimental WMF strength. Unless otherwise indicated, experimental planarians were exposed to 200 μT from 5 minutes postamputation (mpa) to 72 hpa. Experiments were repeated three times (except for untreated controls, which were performed once). Total biological replicates for each condition were as follows: untreated, n = 24; 45 μT, n = 29; and 200 μT, n = 25. For the temporal trials, planarians were exposed beginning at 0 hpa (i.e., 5 mpa), 30 mpa, 1 hpa, 2.5 hpa, 24 hpa, and 48 hpa until the end of the 72-hour period. In addition, planarians were exposed beginning at 0 hpa and ending at 12, 24, or 48 hpa, at which time they were removed from the MagShield box and allowed to continue regenerating in standard incubators (as for untreated controls) until the end of the 72-hour period. Last, planarians were preexposed starting 24 hours before amputation and were either allowed to regenerate with no further exposure or returned to the MagShield box to be further exposed for either 24 or 48 hpa (after which time they continued regenerating in standard incubators until the end of the 72-hour period). Temporal experiments were performed once (except for the 0- to 72-hour exposure, which was repeated three times, and the 30-min to 72-hour exposure, which was repeated twice). Total biological replicates for each condition were as follows: 0 to 72 hours, n = 25; 30 min to 72 hours, n = 19; 1 to 72 hours, n = 12; 2.5 to 72 hours, n = 14; 24 to 72 hours, n = 17; 48 to 72 hours, n = 16; 0 to 12 hours, n = 18; 0 to 24 hours, n = 20; 0 to 48 hours, n = 17; −24 to 24 hours, n = 13; −24 to 48 hours, n = 20; and −24 to 0 hours (amputation), n = 15. For the field strength trials, experiments were performed once, except for 200 μT, which was repeated three times, and 45 μT, which was repeated eight times (as every field strength was run with concurrent controls). Total biological replicates for each condition were as follows: 45 μT, n = 119; 0 μT, n = 30; 100 μT, n = 28; 200 μT, n = 25; 300 μT, n = 18; 400 μT, n = 18; 500 μT, n = 17; and 600 μT, n = 16.

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