HEK293T cells, Huh7 cells, HepG2 cells, and HepG2.2.15 cells obtained from the American Type Culture Collection were maintained at 37°C in 5% CO2 condition. Transfections were performed using PEI (polyethylenimine) for HEK293T cells or Liposomal Transfection Reagent (Hieff Trans) for Huh7 and HepG2 according to the manufacturer’s instructions.

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