Cells of JY21 carrying pKAF131 and pTrc99aMotA were grown in 10 ml of T broth (1% tryptone and 0.5% NaCl) with the appropriate antibiotics [ampicillin (100 μg/ml) and chloramphenicol (170 μg/ml)] and the inducer (0, 2, and 5 μM IPTG). Cells of JY27 carrying pKAF131 were grown in 10 ml of T broth with the appropriate antibiotics [chloramphenicol (170 μg/ml)]. All the cells were grown at 33°C to an OD600 (optical density at 600 nm) between 0.45 and 0.50. Cells of SM1 carrying pBAD33MotBΔplug and pFD313 were grown at 33°C in 10 ml of T broth with the appropriate antibiotics [ampicillin (100 μg/ml) and chloramphenicol (170 μg/ml)] without induction to an OD600 between 0.35 and 0.40 (for about 2 hours and 45 min), and then 0.001% arabinose was added and the cells were grown for an additional 40 min to an OD600 of about 0.53. Cells were harvested by centrifugation at 3500g for 2 min, washed twice in 5 ml of motility medium [10 mM potassium phosphate, 0.1 mM EDTA, 1 mM methionine, and 10 mM lactic acid (pH 7.0)], and resuspended in 2 ml of this medium.

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