The H078.14 UFO-BG trimer structure bound to PGT124 and 35O22 was solved by molecular replacement (MR) using Phaser (103) with the BG505 SOSIP.664 protomer:35O22 component from a previous structure (PDB: 5CEZ) (22), followed by the PGT124 Fab structure (PDB: 4R26) (23) as the MR models. The structures were refined using Phenix (104), with Coot used for model building (105) and MolProbity for structure validation (106). Because of the limited resolution of the datasets, two B-factor groups per residue were used in refinement. The final Rcryst and Rfree values for complex structure are 31.2 and 34.0%. Figures were generated with PyMOL and Chimera (107). In the crystal structure, residues were numbered according to the Kabat definition (108) for the Fabs and according to the HXBc2 system for gp140.

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