The SEC-purified H078.14 UFO-BG trimer complexes were concentrated to ~8 mg/ml before being subjected to extensive crystallization trials at both 4° and 20°C using our automated CrystalMation robotic system (Rigaku) at The Scripps Research Institute (TSRI) (101). Crystals for protein complex containing Fab PGT124 and 35O22 bound to the UFO-BG trimer were obtained from 0.1 M tris (pH 7.4), 0.2 M lithium sulfate, and 6% (w/v) polyethylene glycol 4000, using the sitting drop (0.2 μl) vapor diffusion method, and harvested and cryoprotected with 30% ethylene glycol, followed by immediate flash cooling in liquid nitrogen. The best crystal diffracted to a resolution of 4.60 Å, and diffraction data were collected at the Advanced Photon Source (APS) beamline 23IDB, processed with HKL-2000 (102), and indexed in space group P63 with 98% completeness with the following unit cell parameters: a = b = 127.3 Å and c = 316.3 Å (table S1).

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