Thermal stability of UFO, UFO-BG, and UFO-C trimers expressed in ExpiCHO and 293 F cells and trimer-presenting nanoparticles was measured using a MicroCal VP-Capillary calorimeter (Malvern) in phosphate-buffered saline (PBS) buffer at a scanning rate of 90°C hour−1 from 20° to 120°C. Data were analyzed using the VP-Capillary DSC automated data analysis software.

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