Env proteins and nanoparticles were analyzed by BN-PAGE and stained with Coomassie blue. The protein samples were mixed with G250 loading dye and added to a 4 to 12% bis-tris NuPAGE gel (Life Technologies). BN-PAGE gels were run for 2.5 hours at 150 V using the NativePAGE running buffer (Life Technologies) according to the manufacturer’s instructions.

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