Peripheral blood samples were collected in lithium heparin Vacutainers at day 0, 14 days, 3 months, and 6 months. Peripheral blood mononuclear cells (PBMCs) were isolated via Ficoll-Paque PLUS (GE Healthcare) using LeucoSep Centrifuge Tubes (Greiner), according to the manufacturer’s instructions. Full details are presented in Supplementary Materials and Methods.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.