To charge the success of expression, purification, and reconstitution, LacY, YidC, SecYEG, and SecYEG-YidC samples were Coomassie Blue–stained (fig. S1) or silver-stained (fig. S2) after SDS-PAGE (4 to 12% Bis-Tris Plus gel; Invitrogen, Thermo Fisher Scientific, MA, USA).

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