The microbeads-induced ocular hypertension mouse model was generated according to a previously described method (51) with some modifications. Approximately 8-week-old male mice were used. Sterilized microbeads (Polybead Microspheres; bead diameter, 1 + 6 μm; Polysciences Inc.) in 100% ethanol were washed and resuspended in PBS. After topical anesthesis by instillation with 0.5% proparacaine hydrochloride eye drops, ~4 μl containing 2 μl of equal volume mixture of two-sized microbeads (3 × 106 beads/μl for 6 μm beads and 1.5 × 107 beads/μl for 1 μm beads) and 2 μl of viscoelastic solution [sodium hyaluronate (18 mg/ml), Megacrom; Woojeon Co. Ltd] was injected into the anterior chamber using the Nanoliter 2000 microinjector fitted with a glass capillary pipette. Because the viscoelastic solution entered last into the anterior chamber and pushed microbeads into the peripheral anterior chamber, reflux of microbeads was prevented after withdrawal of the glass capillary pipette. IOP measurements were performed with a rebound tonometer (Tonolab; Tiolat), as previously described (25).

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