Vitreous fluid in mouse eye was sampled using the Nanoliter 2000 microinjector (World Precision Instruments) fitted with a glass capillary pipette. The concentration of Angpt2 in the vitreal fluid was measured using a mouse/rat Angpt2 enzyme-linked immunosorbent assay (ELISA) kit (R&D Systems) according to the manufacturer’s instructions, and the optical density was measured using a Spectra MAX340 plate reader (Molecular Devices).

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