Generation of the laser-induced CNV mouse model was performed according to a previously described method (28) with some modifications (16). Approximately 8-week-old male mice were used for generation of CNV. After dilating pupils with phenylephrine (5 mg/ml) and tropicamide eye drops (5 mg/ml; Santen Pharmaceutical) and instillation with 0.5% proparacaine hydrochloride eye drops (Alcon) for topical anesthesia, laser photocoagulator (Lumenis Inc.) with a slit lamp delivery system was used with a glass coverslip as a contact lens to visualize the retina. Sufficient laser energy (wavelength, 532 nm; power, 250 mW; duration, 100 ms; spot size, 50 μm) was delivered to four locations for each eye (the 3, 6, 9, and 12 o’clock positions of the posterior pole). Only burns that produced a bubble at the time of laser photocoagulation, indicating the rupture of the Bruch’s membrane, were included in this study. Spots containing hemorrhage at the laser site were excluded from the analysis.

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