Recombinant human interleukin-3 (IL-3), IL-6, IL-9, IL-11, stem cell factor (SCF), and Tpo were purchased from PeproTech Inc. BIT 9500 was purchased from STEMCELL Technologies. Anti-CD61 magnetic microbeads and MACS cell separation tools were purchased from Miltenyi. Fluorescein isothiocyanate (FITC)–conjugated anti-CD41, allophycocyanin (APC)–conjugated anti-CD34, and immunoglobulin G antibodies were purchased from BD Biosciences. Anti-GFP (ab290) and corresponding isotype control (ab171870) antibodies were from Abcam. siR-MYB, siR-negative control, miR-486-5p mimics, miR-negative control, CellTracker CM-DiI Dye, and SlowFade Diamond Antifade Mountant with DAPI were purchased from Thermo Fisher Scientific. Alexa 647–conjugated AllStars Negative Control siRNA and the miRNeasy Micro Kit were purchased from Qiagen. All other chemicals were purchased from Sigma-Aldrich.

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