Twenty-four hours after tube formation, vesseloids were fixed in 2% paraformaldehyde and 2% glutaraldehyde (20879.238, VWR) overnight at 4°C. After three washes for 5 min in 0.1 M PB (phosphate buffer), vesseloids were stained with 1% OsO4 in 0.1 M PB before dehydration and impregnation in epoxy resin. Cross sections of the vesseloids were imaged with a Hitachi H7650 electron microscope at high magnification (×25,000 and ×50,000) at the Bordeaux Imaging Center microscopy facility.

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