Paraformaldehyde-fixed and paraffin-embedded samples were cut into 5-μm sections. For immunohistochemical staining, the sections were incubated with rabbit anti-human or mouse MMP-10, mouse anti-human CD8, or rabbit anti-human Reg3a, followed by horseradish peroxidase (HRP)–conjugated anti-rabbit IgG and, later, its substrate diaminobenzidine or Polymer Double Dyeing Detection kit (Mo/HRP + Rb/AP). All the sections were lastly counterstained with hematoxylin and examined using a microscope (Nikon Eclipse 80i, Nikon).

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