Vesseloids were immunostained by an anti-CD31 sheep polyclonal antibody (1:200; AF806, R&D) and either an anti-activated caspase-3 rabbit polyclonal antibody (1:200; G748, Promega) or anti-KI67 monoclonal antibody (1:200; M7240, Dako) before DAPI staining, as described above. We chose two time points, days 1 and 5, after tube formation. Nuclear KI67 and activated caspase-3 signals were quantified using Fiji (, on the same tube length (400 μm), and compared at days 1 and 5.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.