Vesseloids were immunostained by an anti-CD31 sheep polyclonal antibody (1:200; AF806, R&D) and either an anti-activated caspase-3 rabbit polyclonal antibody (1:200; G748, Promega) or anti-KI67 monoclonal antibody (1:200; M7240, Dako) before DAPI staining, as described above. We chose two time points, days 1 and 5, after tube formation. Nuclear KI67 and activated caspase-3 signals were quantified using Fiji (https://fiji.sc/), on the same tube length (400 μm), and compared at days 1 and 5.

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