One-centimeter-long fixed and immunostained vesseloid portions were included in 1% low gelling agarose (A9414, Sigma-Aldrich) within the tailored wells of the UniverSlide (33) filled with DMEM.Confocal images of vascular tubes were acquired with a Nikon Eclipse Ti inverted microscope equipped with a C2Si scan head acquisition software (NIS-Element, NIS). Image analysis was performed with Fiji (https://fiji.sc/).

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