HUVECs and SMCs (C-12205 and C-12511, PromoCell) were maintained in endothelial growth medium 2 (EGM2; C-22111, PromoCell) and SMC growth medium (SMCGM2; C-22062, PromoCell), respectively, under water-saturated 5% CO2 atmosphere at 37°C. Cells were grown in collagen-I–coated flasks (10190103, Thermo Fisher Scientific) and detached at passage 5 for vesseloid formation.

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