The following BM chimeric mice were created: male IL-22−/− BM→female MMP-10−/− mice and male MMP-10−/− BM→female IL-22−/− mice; or male WT BM→female IL-22−/−MMP-10−/− mice, male IL-22−/− BM→female IL-22−/−MMP-10−/− mice, male MMP-10−/− BM→female IL-22−/−MMP-10−/− mice, and male IL-22−/−MMP-10−/− BM→female IL-22−/−MMP-10−/− mice; or male WT BM→female WT mice, male WT BM→female MMP-10−/− mice, male MMP-10−/− BM→female WT mice, and male MMP-10−/− BM→female MMP-10−/− mice. BM cells were collected from the femurs and tibia of donor mice by aspiration and flushing and were suspended in phosphate-buffered saline (PBS) at the concentration of 5 × 107/ml. The BM in recipient mice was ablated with lethal irradiation (8 grays). Then, the animals intravenously received 1.5 × 107 BM cells from donor mice in a volume of 300-μl sterile PBS under the anesthesia. Thereafter, the transplanted BM was allowed to regenerate for 8 weeks before subsequent experimental procedures. To verify successful engraftment and reconstitution of the BM in the host mice, genomic DNA was isolated from tail tissues of each chimera mouse 8 weeks after BM transplantation. Quantitative PCR was performed to detect the Sry gene present in the Y chromosome (primers are shown in table S3) and mouse β2-microglobulin gene as an internal control. The chimeric rates were calculated on the assumption that the ratio of the Sry to β2-microglobulin gene was 100% in male mice. We confirmed that the chimeric rates were consistently higher than 90%. After BM reconstitution was confirmed, mice were infected with bacteria, as described above.

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