Mice. All breeding and experiments were undertaken with review and approval from the Animal Ethical and Experimental Committee of Third Military Medical University (table S2). Through material transfer agreements, C57BL/6 MMP-10−/− mice were obtained from W. C. Parks (University of Washington, USA), and C57BL/6 IL-22−/− mice were obtained from W. Ouyang (Genentech). IL-22−/−MMP-10−/− mice and their littermate control (WT) mice were generated by cross-breeding with IL-22−/− and MMP-10−/− mice. C57BL/6 IL-17A−/− mice and C57BL/6 IFN-γ−/− mice were provided by R. A. Flavell (Yale University, USA). C57BL/6Rag1−/− and C57BL/6CD8−/− mice were obtained from the Jackson Laboratory (Bar Harbor, USA). All mice used in experiments were female except male mice for chimeric experiments and were viral Ab free for pathogenic murine viruses, negative for pathogenic bacteria including Helicobacter spp. and parasites, and were maintained under specific pathogen-free conditions in a barrier-sustained facility and provided with sterile food and water (8).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.