Filling epifluidic devices containing the silver chloranilate reagent with 20 μl of known sodium chloride concentrations (25, 50, 75, and 100 mM) produced the reference color values for calibrating the chloride measurement. Imaging of the resulting product using a Canon EOS Rebel T6i DSLR camera with auxiliary LED lighting yielded the reference images. LAB color values were extracted from the images after correcting the white balance using a color checker (SpyderCheckr 24). The LAB color values from the images of the 25, 50, 75, and 100 mM concentrations formed the reference dial. Printing the reference dial on a 25-μm-thick polyester sheet with adhesive backing (FLEXCon THERMLfilm SELECT 10852) using a commercial ink-jet printer enabled lamination onto the device. Verification of the reference colors was performed by repeating the calibration while using the color reference dial instead of correcting for white balance.

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