Capped mRNAs were synthesized in vitro for pinhead, pinhead-HA, pinhead-GFP, mCherry-CAAX, admp, bmp2b, and gfp from the corresponding linearized plasmids using the mMessage mMachine kit (Ambion). The following MOs were synthesized by Gene Tools and resuspended in nuclease-free water: ph MO (5′-ACTGACAGCAGTAAATCCATAGTCC-3′), admp MO (ad MO; 5′-TGGACAACATTGTAAAGAACATTGC-3′) (14), bmp2b MO (5′-CGCGGACCACGGCGACCATGATC-3′) (30), and chd MO (5′-ATCCACAGCAGCCCCTCCATCATCC-3′) (29). The mRNA and MOs were injected into the yolk, while plasmid DNA was injected into the cytoplasm of one-cell stage embryos.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.