Zebrafish pinhead was cloned into pCS2(+) vectors containing a C-terminal HA or FLAG tag for eukaryotic expression. For Pinhead-GFP, the sequence encoding GFP protein was inserted downstream from the pinhead coding sequence with an “EFLQDIIDGSPGLE” linker separating the fluorescent protein and the Pinhead protein. C-terminal epitope-tagged Admp, Chd, Noggin1, and Bmp1a were cloned in pCS2-Flag, pCS2-HA, or pCS2-Myc vectors. All the resulting constructs were confirmed by sequence analysis.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.