Frozen PFC was homogenized in TBS [25 mM tris-HCl (pH 7.4), 1 mM EDTA, and 1 mM EGTA] containing protease inhibitor cocktail (B14001, Biotool) followed by centrifugation at 100,000g for 30 min at 4°C. The supernatant was collected as TBS fraction, and the pellet was solubilized in 2% SDS and 25 mM tris-HCl (pH 7.4) followed by centrifugation at 100,000g for 30 min at 4°C. The SDS-insoluble pellet was extracted with 70% FA in water. The concentrations of Aβ1-40 (DAB140B, R&D Systems, Minneapolis, MN, USA) and Aβ1-42 (DAB142, R&D Systems, Minneapolis, MN, USA) were measured using enzyme-linked immunosorbent assay (ELISA) kits according to the manufacturer’s instructions. In addition, the levels of IL-1β, IL-6, and TNF-α (BioLegend) in TBS fraction were also quantitatively measured by ELISA. The data were normalized to total protein.

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